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yap inhibitor verteporfin  (ApexBio)
90
ApexBio yap inhibitor verteporfin
Loss of SPTBN1 inhibited autophagy by activating <t>YAP</t> in HCC cells. A and B , QRT-PCR. Huh-7 ( A ) and PLC/PRF/5 ( B ) cells were transiently transfected with siRNA to SPTBN1 or/and YAP for 48 hours and then analyzed. YAP siRNA reversed the expression of autophagy-related genes BECN1 and ATG4B that were downregulated by SPTBN1 knockdown (n = 3; ∗ P < .05, ∗∗ P < .01 vs siCON, # P < .05, ## P < .01 vs siSPTBN1). C and D , Western blot. The decreased ratio of LC3BII/LC3B I protein was reversed by YAP siRNA in Huh-7 ( C ) and PLC/PRF/5 ( D ) cells. Significance of the mean value difference was determined using a Student t test (∗∗ P < .01 vs siCON, ## P < .01 vs siSPTBN1). E , Analysis of autophagy level by Western blot. The decreased ratio of protein LC3BII/ LC3B I was reversed by YAP inhibitor <t>verteporfin</t> in the Huh-7 ( upper ) and PLC/PRF/5 ( lower ) HCC cells. F , The statistical analysis of Western blot in Figure E (n = 3; ∗∗ P < .01 vs siCON, # P < .05, ## P < .01 vs siSPTBN1). G , Autophagy and autophagic flux detection by fluorescence microscopy after GFP-RFP-LC3 LV transfection and HBSS induction for 24 hours. The reduced autophagy spots in response to SPTBN1 knockdown were reversed by YAP siRNA in Huh-7 ( left ) and PLC/PRF/5 ( right ) cells. White bars represent 10 μm.
Yap Inhibitor Verteporfin, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap+inhibitor+verteporfin/yap+inhibitor+verteporfin/pmc08864474-614-6-11
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yap inhibitor verteporfin - by Bioz Stars, 2026-11
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verteporfin  (Bio-Techne corporation)
95
Bio-Techne corporation verteporfin
Loss of SPTBN1 inhibited autophagy by activating <t>YAP</t> in HCC cells. A and B , QRT-PCR. Huh-7 ( A ) and PLC/PRF/5 ( B ) cells were transiently transfected with siRNA to SPTBN1 or/and YAP for 48 hours and then analyzed. YAP siRNA reversed the expression of autophagy-related genes BECN1 and ATG4B that were downregulated by SPTBN1 knockdown (n = 3; ∗ P < .05, ∗∗ P < .01 vs siCON, # P < .05, ## P < .01 vs siSPTBN1). C and D , Western blot. The decreased ratio of LC3BII/LC3B I protein was reversed by YAP siRNA in Huh-7 ( C ) and PLC/PRF/5 ( D ) cells. Significance of the mean value difference was determined using a Student t test (∗∗ P < .01 vs siCON, ## P < .01 vs siSPTBN1). E , Analysis of autophagy level by Western blot. The decreased ratio of protein LC3BII/ LC3B I was reversed by YAP inhibitor <t>verteporfin</t> in the Huh-7 ( upper ) and PLC/PRF/5 ( lower ) HCC cells. F , The statistical analysis of Western blot in Figure E (n = 3; ∗∗ P < .01 vs siCON, # P < .05, ## P < .01 vs siSPTBN1). G , Autophagy and autophagic flux detection by fluorescence microscopy after GFP-RFP-LC3 LV transfection and HBSS induction for 24 hours. The reduced autophagy spots in response to SPTBN1 knockdown were reversed by YAP siRNA in Huh-7 ( left ) and PLC/PRF/5 ( right ) cells. White bars represent 10 μm.
Verteporfin, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap+inhibitor+verteporfin/Verteporfin/custom%405305%4010%2E1101%2F2021%2E02%2E26%2E433068
Average 95 stars, based on 1 article reviews
verteporfin - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier

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Loss of SPTBN1 inhibited autophagy by activating YAP in HCC cells. A and B , QRT-PCR. Huh-7 ( A ) and PLC/PRF/5 ( B ) cells were transiently transfected with siRNA to SPTBN1 or/and YAP for 48 hours and then analyzed. YAP siRNA reversed the expression of autophagy-related genes BECN1 and ATG4B that were downregulated by SPTBN1 knockdown (n = 3; ∗ P < .05, ∗∗ P < .01 vs siCON, # P < .05, ## P < .01 vs siSPTBN1). C and D , Western blot. The decreased ratio of LC3BII/LC3B I protein was reversed by YAP siRNA in Huh-7 ( C ) and PLC/PRF/5 ( D ) cells. Significance of the mean value difference was determined using a Student t test (∗∗ P < .01 vs siCON, ## P < .01 vs siSPTBN1). E , Analysis of autophagy level by Western blot. The decreased ratio of protein LC3BII/ LC3B I was reversed by YAP inhibitor verteporfin in the Huh-7 ( upper ) and PLC/PRF/5 ( lower ) HCC cells. F , The statistical analysis of Western blot in Figure E (n = 3; ∗∗ P < .01 vs siCON, # P < .05, ## P < .01 vs siSPTBN1). G , Autophagy and autophagic flux detection by fluorescence microscopy after GFP-RFP-LC3 LV transfection and HBSS induction for 24 hours. The reduced autophagy spots in response to SPTBN1 knockdown were reversed by YAP siRNA in Huh-7 ( left ) and PLC/PRF/5 ( right ) cells. White bars represent 10 μm.

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Loss of SPTBN1 Suppresses Autophagy Via SETD7-mediated YAP Methylation in Hepatocellular Carcinoma Initiation and Development

doi: 10.1016/j.jcmgh.2021.10.012

Figure Lengend Snippet: Loss of SPTBN1 inhibited autophagy by activating YAP in HCC cells. A and B , QRT-PCR. Huh-7 ( A ) and PLC/PRF/5 ( B ) cells were transiently transfected with siRNA to SPTBN1 or/and YAP for 48 hours and then analyzed. YAP siRNA reversed the expression of autophagy-related genes BECN1 and ATG4B that were downregulated by SPTBN1 knockdown (n = 3; ∗ P < .05, ∗∗ P < .01 vs siCON, # P < .05, ## P < .01 vs siSPTBN1). C and D , Western blot. The decreased ratio of LC3BII/LC3B I protein was reversed by YAP siRNA in Huh-7 ( C ) and PLC/PRF/5 ( D ) cells. Significance of the mean value difference was determined using a Student t test (∗∗ P < .01 vs siCON, ## P < .01 vs siSPTBN1). E , Analysis of autophagy level by Western blot. The decreased ratio of protein LC3BII/ LC3B I was reversed by YAP inhibitor verteporfin in the Huh-7 ( upper ) and PLC/PRF/5 ( lower ) HCC cells. F , The statistical analysis of Western blot in Figure E (n = 3; ∗∗ P < .01 vs siCON, # P < .05, ## P < .01 vs siSPTBN1). G , Autophagy and autophagic flux detection by fluorescence microscopy after GFP-RFP-LC3 LV transfection and HBSS induction for 24 hours. The reduced autophagy spots in response to SPTBN1 knockdown were reversed by YAP siRNA in Huh-7 ( left ) and PLC/PRF/5 ( right ) cells. White bars represent 10 μm.

Article Snippet: Cells were also treated with the YAP inhibitor verteporfin (10 μM, APExBIO, Houston, TX) after SPTBN1 siRNA transfection.

Techniques: Quantitative RT-PCR, Transfection, Expressing, Knockdown, Western Blot, Fluorescence, Microscopy